细胞名称:KPC-胰腺癌-细胞-M(Pten-/-)
是否基因修饰:无,野生型细胞系,未转入任何其他基因
货号:KJ0024-HO-M
来源:C57BL/6j转基因小鼠,雄鼠
相关基因:Pdx1-Cre(负责Cre酶特异性表达), Pten-/-(细胞系基因组中,Pten完全被敲除掉/无蛋白表达),KrasG12D(K-ras基因常见突变,氨基酸序列中第12位甘氨酸突变为天冬氨酸)
是否永生化:可以连续传代30次
是否可以皮下成瘤:是,成功率接近100%(推荐维通利华C57BL/6j小鼠)。一个T25培养皿的细胞量,30%Corning基质胶(Cat NO.356234),合计100uL注入C57BL/6j小鼠皮下,2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。
是否可以原位成瘤:是,成功率接近100%(推荐维通利华C57BL/6j小鼠)。一个T25培养皿的细胞量,30%Corning基质胶(Cat NO.356234),合计50uL注入C57BL/6j小鼠胰腺原位,2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。(如图1所示)
是否有鉴定结果:该细胞系经两家第三方验证,出具STR鉴定报告已经PCR和测序报告。
细胞系培养条件:常规DMEM培养基,含10%FBS,1% P.S.,5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜),个别细胞会呈现泡状。细胞易培养,RPMI-1640培养基也可以培养。
关键词:胰腺癌,胰腺癌转基因小鼠,KPC,KrasG12D, P53/TP53, Pdx1-Cre
Cell line: KPC-pancreatic cancer-cell-F
Genetical modification: No, wild-type cell line, no other genes have been transferred
Cat. No.: KJ0019-HO-M
Background: C57BL/6j transgenic mice, Male mice
Related genes: Pdx1-Cre (responsible for the specific expression of Cre enzyme), Pten-/- (in the cell line genome, Pten is completely knocked out/no protein expression), KrasG12D (a common mutation of the K-ras gene, the 12th glycine in the amino acid sequence mutates to aspartic acid)
Immortalized: It can be continuously passaged 30 times
Subcutaneous tumor: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The amount of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 100uL, is injected subcutaneously into C57BL/6j mice, and a tumor can be formed in 2-3 weeks. Tumors can form in female or male mice.
Tumor in situ: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The number of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 50uL, is injected into the pancreas of C57BL/6j mice in situ, and tumors can form in 2-3 weeks. Tumors can form in female or male mice. (As shown in Figure 1)
Genotyping: The cell line has been verified by two third parties, and STR identification reports and PCR and sequencing reports have been issued. Cell line culture conditions: conventional DMEM culture medium, containing 10% FBS, 1% P.S., 5% CO2, 37℃. 1:3 passage. The cell morphology is shown in Figure 2 (10× objective lens), and some cells will appear bubbly. The cells are easy to culture, and RPMI-1640 culture medium can also be used for culture.
Keywords: pancreatic cancer, pancreatic cancer transgenic mice, KPC, KrasG12D, Pten, Pdx1-Cre