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KP-HCC-Alb-Cre-Pten-M-copGFP+LUC

细胞名称:KP-肝癌-细胞(M)-copGFP+LUC

是否基因修饰:是,非野生型细胞系,通过慢病毒载体(pCDH-CMV-fLuc-EF1a-CopGFP-T2A-Puro-WPRE)转入copGFP和Luciferase,可以用于活体成像和示踪

货号:KJ0012-KP-HO-M-copGFP+LUC

来源:C57BL/6j转基因小鼠,雄鼠

此细胞系源于基因型”Alb-Cre+_pten-/-“小鼠(雄性)

Cell line: KP-HCC-cell (M))-copGFP+LUC

Genetically modified: Yes, Non-wild-type cell line. CopGFP and Luciferase genes were transfected by Lentivirus plasmid(pCDH-CMV-fLuc-EF1a-CopGFP-T2A-Puro-WPRE)

Cat. No.: KJ0012-KP-HO-M-copGFP+LUC

Background: C57BL/6j transgenic mice, Male mice

DMBA诱导小鼠卵巢癌

科研关键词:卵巢癌模型,卵巢癌小鼠模型,DMBA,7,12-二甲基苯并蒽

知识点:1) DMBA 是一种具有致癌活性的多环芳烃 (PAH)。

2) DMBA 多用于啮齿动物模型中诱导肿瘤形成。常见可诱导肿瘤模型包括肺癌,乳腺癌,皮肤癌。

3) DMBA本身是前致癌物,进入体内后主要被肝脏的细胞色素P450酶(尤其是CYP1B1)代谢活化。代谢产生的终末致癌物形式主要是DMBA-3,4-二氢二醇-1,2-环氧化物。这种亲电性的环氧化物能与细胞DNA中的鸟嘌呤碱基共价结合,形成DNA加合物。这种DNA损伤如果未能被有效修复,会导致关键基因(如原癌基因和抑癌基因)发生突变,最终引发细胞恶性转化和肿瘤形成。DMBA对乳腺组织有特别的亲和力,因为乳腺脂肪垫能富集DMBA及其代谢产物。雌性小鼠是最常用的。 因为该模型主要模拟激素反应性乳腺癌。

但我们利用DMBA(Synonyms: 7,12-DMBA; 7,12-Dimethylbenzanthracene)诱导出小鼠卵巢癌。溶解约DMSO中的DMBA直接注入小鼠卵巢中。经过10周的时间诱导出卵巢癌,以下是HE染色如下两幅图。
IHC结果显示,PAX8和AE1AE3呈阳性
如有需要请随时联系我们。目前我们已保存了该肿瘤的PDX,稳定传代。

KPC-PC-Pdx1-Cre-Pten-/–KrasG12D-M

细胞名称:KPC-胰腺癌-细胞-M(Pten-/-)

是否基因修饰:无,野生型细胞系,未转入任何其他基因

货号:KJ0024-HO-M

来源:C57BL/6j转基因小鼠,雄鼠

相关基因:Pdx1-Cre(负责Cre酶特异性表达), Pten-/-(细胞系基因组中,Pten完全被敲除掉/无蛋白表达),KrasG12D(K-ras基因常见突变,氨基酸序列中第12位甘氨酸突变为天冬氨酸)

是否永生化:可以连续传代30次

是否可以皮下成瘤:是,成功率接近100%(推荐维通利华C57BL/6j小鼠)。一个T25培养皿的细胞量,30%Corning基质胶(Cat NO.356234),合计100uL注入C57BL/6j小鼠皮下,2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。

是否可以原位成瘤:是,成功率接近100%(推荐维通利华C57BL/6j小鼠)。一个T25培养皿的细胞量,30%Corning基质胶(Cat NO.356234),合计50uL注入C57BL/6j小鼠胰腺原位,2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。(如图1所示)

是否有鉴定结果:该细胞系经两家第三方验证,出具STR鉴定报告已经PCR和测序报告。

细胞系培养条件:常规DMEM培养基,含10%FBS,1% P.S.,5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜),个别细胞会呈现泡状。细胞易培养,RPMI-1640培养基也可以培养。

关键词:胰腺癌,胰腺癌转基因小鼠,KPC,KrasG12D, P53/TP53, Pdx1-Cre

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Cell line: KPC-pancreatic cancer-cell-F

Genetical modification: No, wild-type cell line, no other genes have been transferred

Cat. No.: KJ0019-HO-M

Background: C57BL/6j transgenic mice, Male mice

Related genes: Pdx1-Cre (responsible for the specific expression of Cre enzyme), Pten-/- (in the cell line genome, Pten is completely knocked out/no protein expression), KrasG12D (a common mutation of the K-ras gene, the 12th glycine in the amino acid sequence mutates to aspartic acid)

Immortalized: It can be continuously passaged 30 times

Subcutaneous tumor: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The amount of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 100uL, is injected subcutaneously into C57BL/6j mice, and a tumor can be formed in 2-3 weeks. Tumors can form in female or male mice.

Tumor in situ: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The number of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 50uL, is injected into the pancreas of C57BL/6j mice in situ, and tumors can form in 2-3 weeks. Tumors can form in female or male mice. (As shown in Figure 1)

Genotyping: The cell line has been verified by two third parties, and STR identification reports and PCR and sequencing reports have been issued. Cell line culture conditions: conventional DMEM culture medium, containing 10% FBS, 1% P.S., 5% CO2, 37℃. 1:3 passage. The cell morphology is shown in Figure 2 (10× objective lens), and some cells will appear bubbly. The cells are easy to culture, and RPMI-1640 culture medium can also be used for culture.

Keywords: pancreatic cancer, pancreatic cancer transgenic mice, KPC, KrasG12D, Pten, Pdx1-Cre

 

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