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细胞名称：KP-肝癌-细胞（M）-copGFP+LUC
是否基因修饰：是，非野生型细胞系，通过慢病毒载体（pCDH-CMV-fLuc-EF1a-CopGFP-T2A-Puro-WPRE）转入copGFP和Luciferase,可以用于活体成像和示踪
货号：KJ0012-KP-HO-M-copGFP+LUC
来源：C57BL/6j转基因小鼠，雄鼠
此细胞系源于基因型”Alb-Cre+_pten-/-“小鼠（雄性）
Cell line: KP-HCC-cell (M)）-copGFP+LUC
Genetically modified: Yes, Non-wild-type cell line. CopGFP and Luciferase genes were transfected by Lentivirus plasmid(pCDH-CMV-fLuc-EF1a-CopGFP-T2A-Puro-WPRE)
Cat. No.: KJ0012-KP-HO-M-copGFP+LUC
Background: C57BL/6j transgenic mice, Male mice
知识点：1） DMBA 是一种具有致癌活性的多环芳烃 (PAH)。
2） DMBA 多用于啮齿动物模型中诱导肿瘤形成。常见可诱导肿瘤模型包括肺癌，乳腺癌，皮肤癌。
3） DMBA本身是前致癌物，进入体内后主要被肝脏的细胞色素P450酶（尤其是CYP1B1）代谢活化。代谢产生的终末致癌物形式主要是DMBA-3,4-二氢二醇-1,2-环氧化物。这种亲电性的环氧化物能与细胞DNA中的鸟嘌呤碱基共价结合，形成DNA加合物。这种DNA损伤如果未能被有效修复，会导致关键基因（如原癌基因和抑癌基因）发生突变，最终引发细胞恶性转化和肿瘤形成。DMBA对乳腺组织有特别的亲和力，因为乳腺脂肪垫能富集DMBA及其代谢产物。雌性小鼠是最常用的。 因为该模型主要模拟激素反应性乳腺癌。
细胞名称：KPC-胰腺癌-细胞-M（Pten-/-）
是否基因修饰：无，野生型细胞系，未转入任何其他基因
货号：KJ0024-HO-M
来源：C57BL/6j转基因小鼠，雄鼠
相关基因：Pdx1-Cre(负责Cre酶特异性表达）, Pten-/-(细胞系基因组中，Pten完全被敲除掉/无蛋白表达），KrasG12D(K-ras基因常见突变，氨基酸序列中第12位甘氨酸突变为天冬氨酸）
是否永生化：可以连续传代30次
是否可以皮下成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计100uL注入C57BL/6j小鼠皮下，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。
是否可以原位成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计50uL注入C57BL/6j小鼠胰腺原位，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。（如图1所示）
是否有鉴定结果：该细胞系经两家第三方验证，出具STR鉴定报告已经PCR和测序报告。
细胞系培养条件：常规DMEM培养基，含10%FBS，1% P.S.，5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜)，个别细胞会呈现泡状。细胞易培养，RPMI-1640培养基也可以培养。
关键词：胰腺癌，胰腺癌转基因小鼠，KPC，KrasG12D, P53/TP53, Pdx1-Cre
Cell line: KPC-pancreatic cancer-cell-F
Genetical modification: No, wild-type cell line, no other genes have been transferred
Cat. No.: KJ0019-HO-M
Background: C57BL/6j transgenic mice, Male mice
Related genes: Pdx1-Cre (responsible for the specific expression of Cre enzyme), Pten-/- (in the cell line genome, Pten is completely knocked out/no protein expression), KrasG12D (a common mutation of the K-ras gene, the 12th glycine in the amino acid sequence mutates to aspartic acid)
Immortalized: It can be continuously passaged 30 times
Subcutaneous tumor: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The amount of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 100uL, is injected subcutaneously into C57BL/6j mice, and a tumor can be formed in 2-3 weeks. Tumors can form in female or male mice.
Tumor in situ: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The number of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 50uL, is injected into the pancreas of C57BL/6j mice in situ, and tumors can form in 2-3 weeks. Tumors can form in female or male mice. (As shown in Figure 1)
Genotyping: The cell line has been verified by two third parties, and STR identification reports and PCR and sequencing reports have been issued. Cell line culture conditions: conventional DMEM culture medium, containing 10% FBS, 1% P.S., 5% CO2, 37℃. 1:3 passage. The cell morphology is shown in Figure 2 (10× objective lens), and some cells will appear bubbly. The cells are easy to culture, and RPMI-1640 culture medium can also be used for culture.
Keywords: pancreatic cancer, pancreatic cancer transgenic mice, KPC, KrasG12D, Pten, Pdx1-Cre
