标签归档：原始细胞系

细胞名称：KPC-胰腺癌-细胞-M（Pten-/-）

是否基因修饰：无，野生型细胞系，未转入任何其他基因

货号：KJ0024-HO-M

来源：C57BL/6j转基因小鼠，雄鼠

相关基因：Pdx1-Cre(负责Cre酶特异性表达）, Pten-/-(细胞系基因组中，Pten完全被敲除掉/无蛋白表达），KrasG12D(K-ras基因常见突变，氨基酸序列中第12位甘氨酸突变为天冬氨酸）

是否永生化：可以连续传代30次

是否可以皮下成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计100uL注入C57BL/6j小鼠皮下，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。

是否可以原位成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计50uL注入C57BL/6j小鼠胰腺原位，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。（如图1所示）

是否有鉴定结果：该细胞系经两家第三方验证，出具STR鉴定报告已经PCR和测序报告。

细胞系培养条件：常规DMEM培养基，含10%FBS，1% P.S.，5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜)，个别细胞会呈现泡状。细胞易培养，RPMI-1640培养基也可以培养。

关键词：胰腺癌，胰腺癌转基因小鼠，KPC，KrasG12D, P53/TP53, Pdx1-Cre

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Cell line: KPC-pancreatic cancer-cell-F

Genetical modification: No, wild-type cell line, no other genes have been transferred

Cat. No.: KJ0019-HO-M

Background: C57BL/6j transgenic mice, Male mice

Related genes: Pdx1-Cre (responsible for the specific expression of Cre enzyme), Pten-/- (in the cell line genome, Pten is completely knocked out/no protein expression), KrasG12D (a common mutation of the K-ras gene, the 12th glycine in the amino acid sequence mutates to aspartic acid)

Immortalized: It can be continuously passaged 30 times

Subcutaneous tumor: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The amount of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 100uL, is injected subcutaneously into C57BL/6j mice, and a tumor can be formed in 2-3 weeks. Tumors can form in female or male mice.

Tumor in situ: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The number of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 50uL, is injected into the pancreas of C57BL/6j mice in situ, and tumors can form in 2-3 weeks. Tumors can form in female or male mice. (As shown in Figure 1)

Genotyping: The cell line has been verified by two third parties, and STR identification reports and PCR and sequencing reports have been issued. Cell line culture conditions: conventional DMEM culture medium, containing 10% FBS, 1% P.S., 5% CO2, 37℃. 1:3 passage. The cell morphology is shown in Figure 2 (10× objective lens), and some cells will appear bubbly. The cells are easy to culture, and RPMI-1640 culture medium can also be used for culture.

Keywords: pancreatic cancer, pancreatic cancer transgenic mice, KPC, KrasG12D, Pten, Pdx1-Cre

细胞名称：KPC-胰腺癌-细胞-F（Pten-/-）

是否基因修饰：无，野生型细胞系，未转入任何其他基因

货号：KJ0024-HO-F

来源：C57BL/6j转基因小鼠，雌鼠

相关基因：Pdx1-Cre(负责Cre酶特异性表达）, Pten-/-(细胞系基因组中，Pten完全被敲除掉/无蛋白表达），KrasG12D(K-ras基因常见突变，氨基酸序列中第12位甘氨酸突变为天冬氨酸）

是否永生化：可以连续传代30次

是否可以皮下成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计100uL注入C57BL/6j小鼠皮下，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。

是否可以原位成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计50uL注入C57BL/6j小鼠胰腺原位，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。（如图1所示）

是否有鉴定结果：该细胞系经两家第三方验证，出具STR鉴定报告已经PCR和测序报告。

细胞系培养条件：常规DMEM培养基，含10%FBS，1% P.S.，5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜)，个别细胞会呈现泡状。细胞易培养，RPMI-1640培养基也可以培养。

关键词：胰腺癌，胰腺癌转基因小鼠，KPC，KrasG12D, P53/TP53, Pdx1-Cre

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Cell line: KPC-pancreatic cancer-cell-F

Genetical modification: No, wild-type cell line, no other genes have been transferred

Cat. No.: KJ0019-HO-F

Background: C57BL/6j transgenic mice, female mice

Related genes: Pdx1-Cre (responsible for the specific expression of Cre enzyme), Pten-/- (in the cell line genome, Pten is completely knocked out/no protein expression), KrasG12D (a common mutation of the K-ras gene, the 12th glycine in the amino acid sequence mutates to aspartic acid)

Immortalized: It can be continuously passaged 30 times

Subcutaneous tumor: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The amount of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 100uL, is injected subcutaneously into C57BL/6j mice, and a tumor can be formed in 2-3 weeks. Tumors can form in female or male mice.

Tumor in situ: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The number of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 50uL, is injected into the pancreas of C57BL/6j mice in situ, and tumors can form in 2-3 weeks. Tumors can form in female or male mice. (As shown in Figure 1)

Genotyping: The cell line has been verified by two third parties, and STR identification reports and PCR and sequencing reports have been issued. Cell line culture conditions: conventional DMEM culture medium, containing 10% FBS, 1% P.S., 5% CO2, 37℃. 1:3 passage. The cell morphology is shown in Figure 2 (10× objective lens), and some cells will appear bubbly. The cells are easy to culture, and RPMI-1640 culture medium can also be used for culture.

Keywords: pancreatic cancer, pancreatic cancer transgenic mice, KPC, KrasG12D, Pten, Pdx1-Cre

细胞名称：ESCC-M

是否基因修饰：无，野生型细胞系，未转入任何其他基因

货号：KJ0041-4-NQO-M

来源：C57BL/6j转基因小鼠，雄鼠, 小鼠经饲喂4-NQO水（200ppm）,间歇性喂水，一周正常饮水，一周4-NQO水，经过7个月以后，解剖发现，小鼠食管多出出现肿瘤。

相关基因：不涉及基因编辑

是否永生化：可以连续传代30次

是否可以皮下成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计100uL注入C57BL/6j小鼠皮下，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。

是否可以原位成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计50uL注入C57BL/6j小鼠食管原位，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。（如图1所示）

是否有鉴定结果：该细胞系经两家第三方验证，出具STR鉴定报告已经PCR和测序报告。

细胞系培养条件：常规DMEM培养基，含10%FBS，1% P.S.，5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜)，大量细胞会呈现泡状。细胞易培养，RPMI-1640培养基也可以培养。

细胞名称：KC-肺癌-细胞-F

是否基因修饰：无，野生型细胞系，未转入任何其他基因

货号：KJ0021-HO-F

来源：C57BL/6j转基因小鼠，雌鼠

相关基因：Sftpc-CreErt2(负责Cre酶特异性表达,需用Tamoxifen诱导，75mg/kg体重，短期内给予5次以上腹腔注射，小鼠II型肺泡细胞会特异性表达Cre酶），KrasG12D(K-ras基因常见突变，氨基酸序列中第12位甘氨酸突变为天冬氨酸）

特别说明：根据我们之前的研究，ERT2的引入是必要的，如果采用sftpc-Cre直接在胚胎开始发挥作用，会出现目标小鼠无法出生，或者出生后身体极度瘦小，脚掌成紫色等症状，寿命不长。

是否永生化：可以连续传代30次

是否可以皮下成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计100uL注入C57BL/6j小鼠皮下，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。

是否可以原位成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计50uL注入C57BL/6j小鼠肺原位，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。（如图1所示）

是否有鉴定结果：该细胞系经两家第三方验证，出具STR鉴定报告已经PCR和测序报告。

细胞系培养条件：常规DMEM培养基，含10%FBS，1% P.S.，5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜)，个别细胞会呈现泡状。细胞易培养，RPMI-1640培养基也可以培养。

关键词：肺癌，肺癌转基因小鼠，KC，KrasG12D, sftpc-CreErt2

细胞名称：KPC-肝内胆管癌-细胞（M）-copGFP+LUC

是否基因修饰：是，非野生型细胞系，通过慢病毒载体（pCDH-CMV-fLuc-EF1a-CopGFP-T2A-Puro-WPRE）转入copGFP和Luciferase,可以用于活体成像和示踪

货号：KJ0012-HO-M-copGFP+LUC

来源：C57BL/6j转基因小鼠，雄鼠

此细胞系源于”KPC-肝内胆管癌-细胞（M）(货号：KJ0012-HO-M，点击链接可见）”

Cell line: KPC-IHCC-cell (M)）-copGFP+LUC

Genetically modified: Yes, Non-wild-type cell line. CopGFP and Luciferase genes were transfected by Lentivirus plasmid(pCDH-CMV-fLuc-EF1a-CopGFP-T2A-Puro-WPRE)

Cat. No.: KJ0012-HO-M-copGFP+LUC

Background: C57BL/6j transgenic mice, Male mice

This cell line is derived from “KPC-IHCC-cell -M (Cat No.:KJ0012-HO-M, click the link to see)”

细胞名称：KPC-胰腺癌-细胞-copGFP+LUC-F

是否基因修饰：是，非野生型细胞系，通过慢病毒载体（pCDH-CMV-fLuc-EF1a-CopGFP-T2A-Puro-WPRE）转入copGFP和Luciferase,可以用于活体成像和示踪

货号：KJ0019-HO-F-copGFP+LUC

来源：C57BL/6j转基因小鼠，雌鼠

此细胞系源于”KPC-胰腺癌-细胞（F）(货号：KJ0019-HO-F，点击链接可见）”

Cell line: KPC-pancreatic cancer-cell-copGFP+LUC-F

Genetical modification: Yes, Non-wild-type cell line. CopGFP and Luciferase genes were transfected by Lentivirus plasmid(pCDH-CMV-fLuc-EF1a-CopGFP-T2A-Puro-WPRE)

Cat. No.: KJ0019-HO-F-copGFP+LUC

Background: C57BL/6j transgenic mice, female mice

This cell line is derived from “KPC-Pancreatic Cancer-Cell (F) (Cat No.:KJ0019-HO-F, click the link to see)”

细胞名称：KPC-肝内胆管癌-细胞（M）

是否基因修饰：无，野生型细胞系，未转入任何其他基因

货号：KJ0012-HO-M

来源：C57BL/6j转基因小鼠，雄鼠

相关基因：Alb-Cre(负责Cre酶特异性表达）, Pten-/-(细胞系基因组中，Pten完全被敲除掉/无蛋白表达），KrasG12D(K-ras基因常见突变，氨基酸序列中第12位甘氨酸突变为天冬氨酸）

是否永生化：可以连续传代30次

是否可以皮下成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计100uL注入C57BL/6j小鼠皮下，2周即可成瘤。雌鼠或者雄鼠都可成瘤。

是否可以原位成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计50uL注入C57BL/6j小鼠胰腺原位，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。（如图1所示）

是否有鉴定结果：该细胞系经两家第三方验证，出具STR鉴定报告已经PCR和测序报告。

细胞系培养条件：常规DMEM培养基，含10%FBS，1% P.S.，5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜)，个别细胞会呈现泡状。细胞易培养，RPMI-1640培养基也可以培养。

关键词：肝内胆管癌，肝内胆管癌转基因小鼠，KPC，KrasG12D, Pten

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Cell line: KPC-IHCC-cell (M)

Genetically modified: No, wild-type cell line, no other genes have been transferred

Cat. No.: KJ0012-HO-M

Background: C57BL/6j transgenic mice, Male mice

Related genes: Alb-Cre (responsible for the specific expression of Cre enzyme), Pten-/- (in the cell line genome, Pten is completely knocked out/no protein expression), KrasG12D (a common mutation of the K-ras gene, the 12th glycine in the amino acid sequence mutates to aspartic acid)

Immortalized: It can be continuously passaged 30 times

Subcutaneous tumor: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The amount of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 100uL, is injected subcutaneously into C57BL/6j mice, and a tumor can be formed in 2` weeks. Tumors can form in female or male mice.

Tumor in situ: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The number of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 50uL, is injected into the pancreas of C57BL/6j mice in situ, and tumors can form in 2-3 weeks. Tumors can form in female or male mice. (As shown in Figure 1)

Genotyping: The cell line has been verified by two third parties, and STR identification reports and PCR and sequencing reports have been issued. Cell line culture conditions: conventional DMEM culture medium, containing 10% FBS, 1% P.S., 5% CO2, 37℃. 1:3 passage. The cell morphology is shown in Figure 2 (10× objective lens), and some cells will appear bubbly. The cells are easy to culture, and RPMI-1640 culture medium can also be used for culture.

Keywords: intrahepatic cholangiocarcinoma/ICC/IHCC, intrahepatic cholangiocarcinoma transgenic mice, ICC transgenic mice, IHCC transgenic mice, KPC, KrasG12D, Pten, Alb-Cre

细胞名称：KPC-胰腺癌-细胞-F（p53-/-）

是否基因修饰：无，野生型细胞系，未转入任何其他基因

货号：KJ0019-HO-F

来源：C57BL/6j转基因小鼠，雌鼠

相关基因：Pdx1-Cre(负责Cre酶特异性表达）, P53-/-(细胞系基因组中，P53完全被敲除掉/无蛋白表达），KrasG12D(K-ras基因常见突变，氨基酸序列中第12位甘氨酸突变为天冬氨酸）

是否永生化：可以连续传代30次

是否可以皮下成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计100uL注入C57BL/6j小鼠皮下，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。

是否可以原位成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计50uL注入C57BL/6j小鼠胰腺原位，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。（如图1所示）

是否有鉴定结果：该细胞系经两家第三方验证，出具STR鉴定报告已经PCR和测序报告。

细胞系培养条件：常规DMEM培养基，含10%FBS，1% P.S.，5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜)，个别细胞会呈现泡状。细胞易培养，RPMI-1640培养基也可以培养。

关键词：胰腺癌，胰腺癌转基因小鼠，KPC，KrasG12D, P53/TP53

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Cell line: KPC-pancreatic cancer-cell (F)

Genetically modified: No, wild-type cell line, no other genes have been transferred

Cat. No.: KJ0019-HO-F

Background: C57BL/6j transgenic mice, female mice

Related genes: Pdx1-Cre (responsible for the specific expression of Cre enzyme), P53-/- (in the cell line genome, P53 is completely knocked out/no protein expression), KrasG12D (a common mutation of the K-ras gene, the 12th glycine in the amino acid sequence mutates to aspartic acid)

Immortalized: It can be continuously passaged 30 times

Subcutaneous tumor : Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The amount of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 100uL, is injected subcutaneously into C57BL/6j mice, and a tumor can be formed in 2-3 weeks. Tumors can form in female or male mice.

Tumor in situ: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The amount of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 50uL, is injected into the pancreas of C57BL/6j mice in situ, and tumors can form in 2-3 weeks. Tumors can form in female or male mice. (As shown in Figure 1)

Genotyping: The cell line has been verified by two third parties, and STR identification reports and PCR and sequencing reports have been issued. Cell line culture conditions: conventional DMEM culture medium, containing 10% FBS, 1% P.S., 5% CO2, 37℃. 1:3 passage. The cell morphology is shown in Figure 2 (10× objective lens), and some cells will appear bubbly. The cells are easy to culture, and RPMI-1640 culture medium can also be used for culture.

Keywords: pancreatic cancer, pancreatic cancer transgenic mice, KPC, KrasG12D, P53/TP53