键词：胰腺癌，胰腺癌转基因小鼠，KPC，KrasG12D, P53/TP53, Pdx1-Cre

名称：KPC-胰腺癌小鼠（p53-/-）

是否基因修饰：是，KrasG12D,P53（TP53）,Pdx1-Cre

货号：KJ0019

来源：C57BL/6j转基因小鼠

相关基因：特异性相对较强，在小鼠胰腺中，Pdx1-Cre(负责Cre酶特异性表达）开始表达, 使得P53-/-（TP53）(导管细胞基因组中，P53完全被敲除掉/无功能性蛋白表达），KrasG12D(K-ras基因常见突变，氨基酸序列中第12位甘氨酸突变为天冬氨酸，Kras蛋白过表达）

成瘤情况介绍：是，成功率接近100%，小鼠四周开始发病，6周龄时腹部隆起，部分小鼠伴有腹水，腹血。小鼠生存周期最长13周，普遍10周-12周。

KPC胰腺癌细胞系已被我们分离（点击链接）。

细胞名称：KPC-胰腺癌-细胞-F（p53-/-）或KPC-胰腺癌-细胞-M

是否基因修饰：无，野生型细胞系，未转入任何其他基因

货号：KJ0019-HO-F或KJ0019-HO-M

来源：C57BL/6j转基因小鼠，雌鼠/雄鼠

相关基因：Pdx1-Cre(负责Cre酶特异性表达）, P53-/-(细胞系基因组中，P53完全被敲除掉/无蛋白表达），KrasG12D(K-ras基因常见突变，氨基酸序列中第12位甘氨酸突变为天冬氨酸）

是否永生化：可以连续传代30次

是否可以皮下成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计100uL注入C57BL/6j小鼠皮下，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。

是否可以原位成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计50uL注入C57BL/6j小鼠胰腺原位，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。如下图

是否有鉴定结果：该细胞系经两家第三方验证，出具STR鉴定报告和PCR,测序报告。

细胞系培养条件：常规DMEM培养基，含10%FBS，1% P.S.，5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜)，个别细胞会呈现泡状。细胞易培养，RPMI-1640培养基也可以培养。

相关KPC小鼠点击链接

关键词：胰腺癌，胰腺癌转基因小鼠，KPC，KrasG12D, P53/TP53, Pdx1-Cre

Cell line: KPC-pancreatic cancer-cell (F) or KPC-pancreatic cancer-cell (M)

Genetical modification: No, wild-type cell line, no other genes have been transferred

Cat. No.: KJ0019-HO-F or KJ0019-HO-M

Background: C57BL/6j transgenic mice, female mice

Related genes: Pdx1-Cre (responsible for the specific expression of Cre enzyme), P53-/- (in the cell line genome, P53 is completely knocked out/no protein expression), KrasG12D (a common mutation of the K-ras gene, the 12th glycine in the amino acid sequence mutates to aspartic acid)

Immortalized: It can be continuously passaged 30 times

Subcutaneous tumor: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The amount of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 100uL, is injected subcutaneously into C57BL/6j mice, and a tumor can be formed in 2-3 weeks. Tumors can form in female or male mice.

Tumor in situ: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The number of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 50uL, is injected into the pancreas of C57BL/6j mice in situ, and tumors can form in 2-3 weeks. Tumors can form in female or male mice. (As shown in Figure 1)

Genotyping: The cell line has been verified by two third parties, and STR identification reports and PCR and sequencing reports have been issued. Cell line culture conditions: conventional DMEM culture medium, containing 10% FBS, 1% P.S., 5% CO2, 37℃. 1:3 passage. The cell morphology is shown in Figure 2 (10× objective lens), and some cells will appear bubbly. The cells are easy to culture, and RPMI-1640 culture medium can also be used for culture.

Keywords: pancreatic cancer, pancreatic cancer transgenic mice, KPC, KrasG12D, P53/TP53, Pdx1-Cre