细胞名称：KPC-肝内胆管癌-细胞（M）或 KPC-肝内胆管癌-细胞（F）
是否基因修饰：无，野生型细胞系，未转入任何其他基因
货号：KJ0012-HO-M或KJ0012-HO-M
来源：C57BL/6j转基因小鼠，分别来自雄鼠和雌鼠
相关基因：Alb-Cre(负责Cre酶特异性表达）, Pten-/-(细胞系基因组中，Pten完全被敲除掉/无蛋白表达），KrasG12D(K-ras基因常见突变，氨基酸序列中第12位甘氨酸突变为天冬氨酸）
是否永生化：可以连续传代30次
是否可以皮下成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计100uL注入C57BL/6j小鼠皮下，2周即可成瘤。雌鼠或者雄鼠都可成瘤。
是否可以原位成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计50uL注入C57BL/6j小鼠肝原位，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。
是否有鉴定结果：该细胞系经两家第三方验证，出具STR鉴定报告已经PCR和测序报告。
细胞系培养条件：常规DMEM培养基，含10%FBS，1% P.S.，5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜)，个别细胞会呈现泡状。细胞易培养，RPMI-1640培养基也可以培养。
关键词：肝内胆管癌，肝内胆管癌转基因小鼠，KPC，KrasG12D, Pten
Cell line: KPC-IHCC-cell (M) or KPC-IHCC-cell (F)
Genetical modification: No, wild-type cell line, no other genes have been transferred
Cat. No.: KJ0012-HO-M or KJ0012-HO-F
Background: C57BL/6j transgenic mice, derived from Male mouse or Female mouse
Related genes: Alb-Cre (responsible for the specific expression of Cre enzyme), Pten-/- (in the cell line genome, Pten is completely knocked out/no protein expression), KrasG12D (a common mutation of the K-ras gene, the 12th glycine in the amino acid sequence mutates to aspartic acid)
Immortalized: It can be continuously passaged 30 times
Subcutaneous tumor: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The amount of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 100uL, is injected subcutaneously into C57BL/6j mice, and a tumor can be formed in 2` weeks. Tumors can form in female or male mice. (As shown in Figure 1)
Tumor in situ: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The number of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 50uL, is injected into the pancreas of C57BL/6j mice in situ, and tumors can form in 2-3 weeks. Tumors can form in female or male mice.
Genotyping: The cell line has been verified by two third parties, and STR identification reports and PCR and sequencing reports have been issued. Cell line culture conditions: conventional DMEM culture medium, containing 10% FBS, 1% P.S., 5% CO2, 37℃. 1:3 passage. The cell morphology is shown in Figure 2 (10× objective lens), and some cells will appear bubbly. The cells are easy to culture, and RPMI-1640 culture medium can also be used for culture.
Keywords: intrahepatic cholangiocarcinoma/ICC/IHCC, intrahepatic cholangiocarcinoma transgenic mice, ICC transgenic mice, IHCC transgenic mice, KPC, KrasG12D, Pten, Alb-Cre
键词：胰腺癌，胰腺癌转基因小鼠，KPC，KrasG12D, P53/TP53, Pdx1-Cre
名称：杂合子KPC-胰腺癌小鼠（p53+/-）
是否基因修饰：是，KrasG12D,P53（TP53）,Pdx1-Cre
货号：KJ0019
来源：C57BL/6j转基因小鼠
相关基因：特异性相对较强，在小鼠胰腺中，Pdx1-Cre(负责Cre酶特异性表达）开始表达, 使得P53-/-（TP53）(导管细胞基因组中，P53在基因组中部分不完全删除），KrasG12D(K-ras基因常见突变，氨基酸序列中第12位甘氨酸突变为天冬氨酸，Kras蛋白过表达）
成瘤情况介绍：是，成功率接近100%，小鼠12周开始发病，16周左右腹部开始隆起，部分小鼠伴有腹水，腹血。小鼠生存周期可以长达20周。
Keywords: Pancreatic cancer, Pancreatic cancer transgenic mouse, KPC, KrasG12D, P53/TP53, Pdx1-Cre
Name: Heterozygous-KPC-Pancreatic Cancer Mouse (p53+/-)
Genetically Modified: Yes, KrasG12D, P53 (TP53), Pdx1-Cre
Catalog Number: KJ0019
Origin: C57BL/6J transgenic mouse
Related Genes: High specificity; in the mouse pancreas, Pdx1-Cre (responsible for specific Cre recombinase expression) is activated, resulting in P53-/- (TP53) (incomplete knockout of P53 in the ductal cell genome, no functional protein expression), and KrasG12D (a common K-ras mutation where glycine at position 12 is substituted with aspartic acid, leading to Kras protein overexpression).
Tumorigenesis Overview: Yes, with a near 100% success rate. Tumor onset occurs around 12 weeks of age, with abdominal distension observed by 16 weeks, accompanied by ascites and abdominal hemorrhage in some mice. Maximum survival period is 20 weeks.