KPC肝内胆管癌细胞系（Pten)

细胞名称：KPC-肝内胆管癌-细胞（M）或 KPC-肝内胆管癌-细胞（F）

是否基因修饰：无，野生型细胞系，未转入任何其他基因

货号：KJ0012-HO-M或KJ0012-HO-M

来源：C57BL/6j转基因小鼠，分别来自雄鼠和雌鼠

相关基因：Alb-Cre(负责Cre酶特异性表达）, Pten-/-(细胞系基因组中，Pten完全被敲除掉/无蛋白表达），KrasG12D(K-ras基因常见突变，氨基酸序列中第12位甘氨酸突变为天冬氨酸）

是否永生化：可以连续传代30次

是否可以皮下成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计100uL注入C57BL/6j小鼠皮下，2周即可成瘤。雌鼠或者雄鼠都可成瘤。

是否可以原位成瘤：是，成功率接近100%（推荐维通利华C57BL/6j小鼠）。一个T25培养皿的细胞量，30%Corning基质胶（Cat NO.356234）,合计50uL注入C57BL/6j小鼠肝原位，2-3周即可成瘤。雌鼠或者雄鼠都可成瘤。

是否有鉴定结果：该细胞系经两家第三方验证，出具STR鉴定报告已经PCR和测序报告。

细胞系培养条件：常规DMEM培养基，含10%FBS，1% P.S.，5% CO2, 37℃。1:3传代。细胞形态如图2所示(10×物镜)，个别细胞会呈现泡状。细胞易培养，RPMI-1640培养基也可以培养。

关键词：肝内胆管癌，肝内胆管癌转基因小鼠，KPC，KrasG12D, Pten

Cell line: KPC-IHCC-cell (M) or KPC-IHCC-cell (F)

Genetical modification: No, wild-type cell line, no other genes have been transferred

Cat. No.: KJ0012-HO-M or KJ0012-HO-F

Background: C57BL/6j transgenic mice, derived from Male mouse or Female mouse

Related genes: Alb-Cre (responsible for the specific expression of Cre enzyme), Pten-/- (in the cell line genome, Pten is completely knocked out/no protein expression), KrasG12D (a common mutation of the K-ras gene, the 12th glycine in the amino acid sequence mutates to aspartic acid)

Immortalized: It can be continuously passaged 30 times

Subcutaneous tumor: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The amount of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 100uL, is injected subcutaneously into C57BL/6j mice, and a tumor can be formed in 2` weeks. Tumors can form in female or male mice. (As shown in Figure 1)

Tumor in situ: Yes, the success rate is close to 100% (Charles River/维通利华 C57BL/6j mice are recommended). The number of cells in a T25 culture dish, 30% Corning matrix gel (Cat NO.356234), a total of 50uL, is injected into the pancreas of C57BL/6j mice in situ, and tumors can form in 2-3 weeks. Tumors can form in female or male mice.

Genotyping: The cell line has been verified by two third parties, and STR identification reports and PCR and sequencing reports have been issued. Cell line culture conditions: conventional DMEM culture medium, containing 10% FBS, 1% P.S., 5% CO2, 37℃. 1:3 passage. The cell morphology is shown in Figure 2 (10× objective lens), and some cells will appear bubbly. The cells are easy to culture, and RPMI-1640 culture medium can also be used for culture.

Keywords: intrahepatic cholangiocarcinoma/ICC/IHCC, intrahepatic cholangiocarcinoma transgenic mice, ICC transgenic mice, IHCC transgenic mice, KPC, KrasG12D, Pten, Alb-Cre